The price of our newly developed tranfection reagent, NeoFectin, is 40% lower than our major competitor

Neofectin^TM is a newly developed, easy-to-use transfection reagent for DNA deliveries. Neofectin^TM, combines two proven gene delivery platforms, polyethylenimine (PEI) and cationic lipid based formulations. PEI polymer is known for its efficiency in condensation of DNA plasmid and PEI formulations can facilitate DNA uptake by cells and protect DNA from degradation in endosomes. Cationic liposome is effective in initiating lipid mixing for fusion of membrane and liposome formulations can facilitate release of DNA from endosomes. Our proprietary formulation methods successfully integrate these two platform technologies and produce a much improved transfection agent, Neofectin^TM. Neofectin^TM delivers superior performance with no toxicity commonly known for liposome based formulations and has been evaluated on many mammalian cells, include many difficult-to-transfect primary cells.

Product List:

Catalog_No.	Description	Unit	Price
NF0866-1	NeoFectinTM, Maximum strength for DNA in vitro tranfection, sufficient for 20 transfection (24 well plate)	0.1mL	$29.00
NF0866-2	NeoFectinTM, Maximum strength for DNA in vitro tranfection, sufficient for 200 transfection (24 well plate)	1mL	$165.00
NF0877-1	NeoFectinTM LT , extreme low toxicity for DNA in vitro tranfection, sufficient for 20 transfection (24 well plate)	0.1mL	$29.00
NF0877-2	NeoFectinTM LT , extreme low toxicity for DNA in vitro tranfection, sufficient for 200 transfection (24 well plate)	1mL	$165.00

Key Features:

• Simple and efficient mix-and-apply protocol.
• Stable formulation with long shelf life.
• Superior and consistent performance with low cytotoxicity.
• Cost effective comparing to key competitive products, such as Lipofectamine 2000.
• Applicable to many cell lines (for the list of cell lines tested with Neofectin^TM).

Cell Lines	Transfection Efficiency(% GFP)	Cell Lines	Transfection Efficiency(% GFP)
3LL	51%	K562	22%
B16-F10	72%	L929	52%
BAEC	52%	MCF-7	51%
BHK-21	86%	MDCK	52%
Ca Ski	78%	Neuro2A	72%
CaCo2	32%	NIH 3T3	88%
CHO	83%	PC12	34%
HCS-2/8	42%	SH-SY5Y	14%
HEK-293	80-90%	SiHa	62%
HeLa	81%	SKOV3	52%
HLMEC	50-65%	HUVEC	51%
H-MVEC	49%	IGROV1	21%
Huh-7D12	22%	Jurkat	5%-15%
ATT20	38%	6CSFMEo	73%
SK-N-SH	20%	WEHI 231	25%
McArdle 7777	60%	SAOS-2	52%
Hep3D	78%	SN56	69%
SHEP	71%	MC3T3-E1	80%
MDA-MB-231	38%	BT474	46%
3T3-442A	10%	C2C12	46%
COS-7	60-70%	Primary mouse keratinocytes	20-30%
CV-1	53%	Primary human pre-adipocytes	20-30%
D 407	62%	Primary human skin fibroblasts	15%
DHD Pro.b	51%	Primary mouse embryonic fibroblast	10%
LS180	46%	Primary melanocyte	15-20%
A549	68%	Hela-S3	78%